Brazil right-back Dani Alves was ruled out of the World Cup on Friday after suffering a knee injury playing for French champions Paris Saint-Germain, the Brazilian football federation (CBF) said.
The 35-year-old suffered the injury playing in Tuesday's French Cup final against Les Herbiers, coming off four minutes from the end as PSG beat the minnows 2-0.
The World Cup, where Brazil are aiming for a sixth title, starts in Russia on June 14.
Brazilian legend and three-time World Cup-winner Pele tweeted: “I am so sad to hear this.
Footballers hate missing games but I know first-hand that missing #WorldCup games is the worst feeling.”
The injury to Alves is the second major blow for Brazil who are still sweating on the recovery from foot surgery of star striker Neymar who hasn't played since March.
Alves has played 107 times for Brazil since making his debut in 2006 and looked a certain starter for Tite's team at the World Cup finals in Russia where Brazil are grouped with Switzerland, Costa Rica and Serbia.
Tite is due to name his squad on Monday.
PSG coach Unai Emery said he sympathised with Alves missing what would have been his last World Cup.
“The doctors have studied what is best for Dani Alves. I am really sorry for him,” said Emery.
Alves, a former Barcelona and Juventus star, has won 38 trophies in his career -- a new record secured by PSG's French Cup triumph on Tuesday.
In his 17-year career, he won his first trophy with boyhood club Esporte Clube de Bahia followed by five at Sevilla, 23 with Barcelona, two at Juventus and four so far at PSG.
For Brazil, Alves has secured three winner's medals.
Worryingly for Tite, there is no outstanding natural replacement for Alves in the right-back slot.