Fellow midfielders Rafinha and Arda Turan, and defender Aleix Vidal are all missing through injury.
Barcelona, who are second behind Real Madrid in the La Liga title race, became the first team in Champions League history to overhaul a first-leg deficit of four goals when they beat French champions PSG.
Sergio Roberto's late winner set up a rematch of the 2015 final against Juventus, when the Catalans won 3-1 to be crowned European champions for a fifth time.
Juventus are undefeated in 21 European home matches and have won their previous 32 Serie A games at Juventus Stadium.
Juve have won two of their four home games in this season's Champions League, drawing the other two.
Barcelona are in the Champions League quarter-finals for a record 10th season in a row.
Barca have lost two of their past three away games in the Champions League.
Juventus beats FC Barcelona 3-0
Goal Scorers for Juventus - Paulo Dybala(07 and 22) and Giorgio Chiellini(55)
Paulo Dybala scored twice as Juventus took charge of their Champions League QF tie with Barcelona courtesy of a commanding first-leg display in Turin.
The Argentine forward curled home both of his goals before the break, the first from an angle inside the box and the second from a central position on the edge.
Juve turned a dominant lead into one that should see them go on and win the tie when Giorgio Chiellini showed strength and guile to steer home a header from a corner.
For the second European round running, Barca - who were as defensively suspect as they were in losing 4-0 to Paris St-Germain in the first leg of their last-16 tie - must recover from a heavy away defeat to progress.
Juventus are unbeaten in their last 18 Champions League home games (W11 D7 L0), their longest ever run without a defeat in the competition on home soil. Their last home defeat in the competition was versus Bayern Munich in April 2013 (0-2).
Juventus have now won 16 successive home matches in all competitions and are unbeaten in 48 games there (W42 D6 L0). Their last home defeat in all comps came against Udinese in August 2015.
Massimiliano Allegri has equalled Juventus' longest winning streak in the Champions League (five games - previously done by Fabio Capello in 2004-05 and Antonio Conte in 2012-13).
Dybala's first two goals in 2016-17 for Juventus were scored away from home, but his 14 goals since then have all been scored at the Juventus Stadium.
Barcelona have lost four of their past five Champions League away games in the knock-out stages (W1 D0 L4), conceding 12 goals in these games.